Sample requirements
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Samples may be submitted on Tuesdays and Wednesdays only. Samples that will be received on Thursdays and Fridays will be considered submissions for the following week.
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Clients may opt to be responsible for delivering their samples to DSCF. Otherwise, DSCF will charge an additional flat rate delivery fee of:
Turnaround time
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Results will be given on Friday of the same week of the submission.
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Results may be given sooner for 96-well plate submissions.
Sample container formats accepted
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For primers:
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1.5-mL microtubes
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For samples:
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1.5-mL microtubes
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0.2-mL PCR tubes
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8- or 12-tube PCR strip with cap
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96-well PCR plate (ideally skirted) with pierceable aluminum sealing film
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Submission​
Note:
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- We accept unpurified PCR products/plasmids for an additional fee
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For Extract2Seq 16s rRNA gene Bacterial Identification, provide pure bacterial isolates in agar plate/broth tubes (for broth cultures, provide at least 2mL of sample.
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Important Reminders!
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Do not exceed the specified concentration ranges.
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Ensure that there are no leftover PCR reagents (dNTPs, primers) or protein contamination in your sample. As a guide, the A260/A280 ratio of DNA samples should be at ~1.8-2.0.
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Ensure that there is enough primer provided for the ordered reactions and any possible re-runs.
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If using TE buffer to dilute DNA samples, keep salt concentration at a minimum of 10 mM Tris containing 125 mM KCl or lower.
Sample Quality Requirements
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Please refer to the following agarose gel image below:
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Figure 1. Agarose gel image of DNA template/samples with good (A) and bad (B) quality
A
Note:
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Samples should appear as a single, intense and sharp band on the gel.
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Samples should not contain primer dimers.