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Important Reminders!

  • Do not exceed the specified size range and concentration range

  • Ensure that there is no protein contamination in your sample

  • If using TE buffer to dilute samples, keep salt concentration at a minimum: 10 mM Tris containing 125 mM KCl or lower for DNA and 10 mM Tris containing 1 mM EDTA for RNA

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Sample requirements

  • Provide at least 3 μL of sample

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Sample container formats accepted

  • 0.2 mL PCR tube with cap 

  • 0.2 mL 8- or 12-tube PCR strip with cap

 

Submission

  • Samples may be submitted from Monday to Friday until 3 PM.

  • Clients may opt to be responsible for delivering their samples to DSCF. Otherwise, DSCF will charge an additional flat rate delivery fee of:​

QuBit 2.0 Fluorometry

Turnaround time

  • Results will be given on the same day of submission.

MultiNA Microchip Electrophoresis

Sample requirements

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  • DNA

    • Size must be from 25 bp to 2,500 bp only; no genomic DNA accepted

    • Provide at least 10 µL sample with a concentration of 20-50 ng/µL

    • Use nuclease-free water or TE buffer to dilute samples if necessary

  • RNA

    • Up to 28S rRNA (4.7 to 5.0 knt) accepted

    • Provide at least 5 µL sample with a concentration of 50-250 ng/µL

    • Use Invitrogen’s THE RNA Storage Solution (recommended) or TE buffer to dilute samples if necessary

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Sample container formats accepted

  • 8- or 12-tube PCR strip with cap

  • 96-well PCR plate with pierceable aluminium sealing film

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Submission​

  • Samples may be submitted on Tuesdays and Wednesdays only. Samples that will be received on Thursdays and Fridays will be considered submissions for the following week.

  • Clients may opt to be responsible for delivering their samples to DSCF. Otherwise, DSCF will charge an additional flat rate delivery fee of:

Turnaround time

  • Results will be given on Thursday of the same week of the submission.

  • Results may be given sooner for 96-well plate submissions (at least 80 samples).

© 2018 by DNA Sequencing Core Facility, Philippine Genome Center

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